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小鼠肿瘤坏死因子α(TNF-α)试剂盒(ELISA)
l 本试剂盒用于体外定量检测血清、血浆、组织匀浆及相关液体样本中小鼠肿瘤坏死因子α(TNF-α)的含量。
l 有效期:6个月
l 保存条件:2-8℃
l 本试剂盒仅供科研使用,不得用于临床诊断
实验原理
试剂盒采用双抗体一步夹心法酶联免疫吸附试验(ELISA)。往预先包被小鼠肿瘤坏死因子α(TNF-α)捕获抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的小鼠肿瘤坏死因子α(TNF-α)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品浓度。
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